Welcome to my homepage

Name:

Stefan VILCEK

Born:

December 19??

Hobbies:

Popularization of science, hiking, mountains, photography, backpacker

Life philosophy:

To be useful as much as possible

Contact:

The University of veterinary medicine and pharmacy in Košice
Department of epizootiology and preventive veterinary medicine
Komenskeho 73, SK-041 81 Kosice, Slovakia
E-mail: vilcek@uvm.sk
Phone: +421 915 984654; Fax: +421 55 2981011

Graduation
Occupation
Membership
Scientific visits of international laboratories
Collaboration with experts abroad
Grants
The most significant achievements
Teaching activities
Selected presentations on international conferences
Selected references
The most frequently cited paper
Pictures from nature 1, Pictures from nature 2
Pictures from the world I, Pictures from the world II, Pictures from the world III

Graduation:

Ing. - The Czech Technical University, Praque (branch nuclear chemistry) - 1974
Ph.D. - Comenius University, Bratislava - 1981
PhD thesis: Preparation of ^99mTc-labelled compounds using different reducing agents
Postgradual study: Four semesters of Genetic Engineering at Comenius University, Bratislava - 1987-1988
Associate Professor - University of P. J. Safarik, Kosice - 1994
Habilitation work: The application of molecular-genetic techniques for detection of some viruses
DrSc. (Doctor of Science) - Institute of Virology, Slovak Academy of Sciences, Bratislava - 1999
Title of work: "Genetic idetification and characterization of viruses in some farm animals"
Professor - University of Veterinary Medicine, Kosice - 2005

Occupation:

Faculty of Human Medicine, Kosice (1974 - 1980) - scientific worker
University of Veterinary Medicine, Kosice (1981 - ...) - principal scientific investigator
Research Institute of Experimental Medicine, Kosice (1986 - 2003) - Head of Laboratory of Genetic Manipulations

Member of:

Czechoslovak Microbiological Society
ESVV (European Society for Veterinary Virology)
Editorial Board of journal "Vesmír"
Editorial Board of journal "Folia Veterinaria" (2005 - )
Editorial Board of journal "Virus Genes" (2010 - )
Editoarial Board of journal"Veterinarni medicina (Praque)" (2011 - )
Editorial Board of journal"Frontiers in Genomic Lifestock" (2011 - )
Scientific Council of University of Veterinary Medicine in Kosice (1999 - 2002, 2003 - 2007, 2007 - 2011)
Scientific Council of Faculty of Science, University of P.J. Safarik in Kosice (2007 - 2011)
Slovak Scientific Grant Agency (2002 - 2008)
Subcommission of Slovak State Scientific Program (2002 - 2006)
ESVV (European Society for Veterinary Virology) Board Member (2003 - 2006, 2006 - 2009) (http://esvv.eu)
Bioethics Committee of UNESCO in The Slovak Republic
Member of state committee for the defence of doctor scientific works (DrSc.) in the field molecular biology
Member of scientific collegiums for molecular biology and genetics of Slovak Academy of Sciences in Bratislava

Scientific visits of international laboratories:

Moscow Veterinary University, October - November 1988
(Application of molecular-genetic techniques in veterinary medicine)
The Moredun Research Institute, Edinburgh, September 1992 - August 1993
(Development of PCR assays for detection of BHV 1 and pestiviruses)
The National Veterinary Institute, Uppsala, October - December 1993
(Development of RT-PCR assay for detection of BRSV)
The National Veterinary Institute, Uppsala, April - December 1995
(Molecular epidemiology of CSF)
The National Veterinary Institute, Uppsala, April - July 1997
(Study of pestivirus genome)
Central Veterinary Laboratory, Weybridge, UK, October - November 1997
(Molecular epidemiology of BVD in UK)
Central Veterinary Laboratory, Weybridge, UK, September - November 1998
(Study of BVDV genome, BVDV in vaccines, RT-PCR for BDV)
Veterinary Administrative Office of Lower Austria, May 1999
(Molecular-genetic methods in eradication program against BVD/MD)
Central Veterinary Laboratory, Weybridge, UK, September – December 1999
(Genetic typing of BVDV from different European countries)
Veterinary Seum and Vaccine Research Institute, Cairo, Egypt – January 2000
(Genetic typing of BVDV in Egypt)
Central Veterinary Laboratory, Weybridge, UK, September – January 1999
(Genetic typing of BVDV from different European countries – cont.)
Institute of Virology, Hannover Veterinary School, Hannover, Germany – Februar 2000
(Establishement of the collaboration on genetic typing of BVDV)
National Animal Disease Center, Ames, Iowa, USA, September - November 2000
(Genetic analysis of BVDV2 and new pestivirus from antelope)
Investigation Center for Animal Health (CISA-INIA), Valdeolmos, Spain, October - November 2002
(Correlation of the antigenic and genetic data for CSFV strains)
Investigation Center for Animal Health (CISA-INIA), Valdeolmos, Spain, May 2003
(Correlation of the antigenic and genetic data for CSFV strains)
Universidad Nacional de Colombia, Bogota, Colombia - September 2003
(Workshop on IBR and BVDV infections, establishment of scientific collaboration)
Institute of Virology, Hannover Veterinary School, Hannover, Germany - December 2003
(Real time RT-PCR)
The National Veterinary Institute, Uppsala, February 2004
(Establishment of collaboration on genetic analysis of coronaviruses)
The Moredun Research Institute, Edinburgh, June 2005
(Genetic analysis of a pestivirus isolated from chamois)
AFSSA, Unite Genetique Virale et Biosecurite, Ploufragan, France, October 2006
(Real-time PCR and microarray technique for detection and analysis of cell-PCV2 interaction)
Pardue University, School of Veterinary Medicine, West Lafayette, Indiana, USA, May 2007
(Establishment of collaboration on genetic analysis of viruses)
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Bhopal, India, October 2008
(Genetic analysis of pestiviruses)
AFSSA, Ploufragan, France, January 2011
(Microarray terchnique for study of host-virus interaction at the genetic level)

Collaboration with experts abroad (in alphabetic order):

Prof. BAUMGARTNER Walter, Prof. MOSTL Karin - Veterinary University, Vienna, Austria
Prof. BELAK Sandor – National Veterinary Institute, Uppsala, Sweden
Dr. BLANCHARD Yannick – AFSSA, Ploufragan, France
Dr. GERILOVYCH Anton – Institute of Experimental and Clinical Veterinary Medicine, Kharkov, Ukraine
Dr. GREISER-WILKE Irene – School of Veterinary Medicine, Hannover, Germany
Dr. JESTIN Andre – AFSSA, Ploufragan, France
Dr. MAHONY Timothy - Agency for Food and Fibre Sciences, Brisbane. Australia
Dr. MISHRA Niranjan - Indian Veterinary Research Institute, Bhopal, India
Dr. NETTLETON Peter – Moredun Research Institute, Edinburgh, Scotland
Dr. PATON David – Central Veterinary Laboratory, Weybridge, England; Institute for Animal Health, Pirbright, England
Prof. PETERHANS Ernst – University of Bern, Institute of Veterinary Virology, Bern,Switzerland
Dr. POGRANICHNIY Roman – Purdue University, School of Veterinary Medicine, West Lafayette, Indiana, USA
Dr. RIDPATH Julia – National Animal Disease Center, Ames, Iowa, USA
Dr. ROSMANITH Wigbert – Veterinary Office, Gov. of Lower Austria, St. Polten, Austria

Grants:

International grants:

Understanding and combating porcine reproductive and respiratory syndrome in Europe
Project leader: Dr. Ait-Ali Tahar, University of Ediburgh, UK
Project leader in Slovakia: Vilcek, S.
Period:2011-2013
COST Action č. FA0902

Epidemiolohy of bovine viral diarrhea in Ukraine: Development of a control program
Project leaders: Ernst Peterhans (Switzerland), Stefan Vilcek (Slovakia), Anton Gerilovych (Ukraine)
Period: 2010-2012
SCOPUS project No. IZ73Z0_128050/1

PCVD: Towards improved food quality and safety within new member states and associated candidate countries
Project leader: Dr. Gordon Allan, University Belfast
Project leader in Slovakia: Vilcek, S.
Period 2006-2008
SSA of FP6, No 518432

BVDV control in Europe
Project leader: Paul S. Valle (Norway), Partner 15: Vilcek, S. (Slovakia)
Period 2002-2005
Thematic network of FP5, QLRT - 2001-01573

Development of molecular-genetic methods for the detection of pestiviruses.
Project leaders: Vilcek, S. (Slovakia); Greiser-Wilke, I. (Germany)
Period: 2002-2003
Slovak-German project

Molecular-genetic characterization, detection and discrimination of pestiviruses.
Project leaders: Vilcek, S. (Slovakia); Paton, D.J. (UK)
Period: 1997-2000
The Welcome Trust grant, No. 049956/Z/97/Z/JMW/JPS/CG

Grants in Slovakia:

Classical, genomic and proteomic approach in investigation of economically important viral infections of farm animals.
Project leader: Vilcek, S.
Period: 2011-2014
Project No. APVV-0379-10

Epizootiology, immunity and genetic analysis of agents for important and other virus infections in pigs
Project leader: Vilcek, S.
Period: 2011-2013
Grant VEGA, No. 1/0704/11

INFEKTZOON – Center of excellence for infectious diseases and zoonosis
Period: 2009 /- 2011
Scientific leader: Vilcek, S.
Project supported from structural founding of EU

Variability of viral genomes, molecular diagnostics and epidemiology of animal infections.
Project leader: Vilcek, S.
Period: 2006-2010
Project no: APVV-20-019605

Identification of the agents of infectious diseases of farm animals on the base of real-time PCR and analysis of virus genomes.
Project leader: Vilcek, S.
Period: 2008-2010
Grant VEGA, No 1/0389/08

Genetic analysis, diversity and typing of selected viruses with (+)ssRNA genome
Period 2005-2007
Project leader: Vilcek, S.
Grant VEGA, No 1/2330/05

Genomics of animal virus infections
Project leader: Vilcek, S.
Period: 2003-2005
State program No SP51/0280800/0280803

Genetic diagnosis, analysis of viruses and epidemiological studies important viral infections of farm animals.
Project leader: Vilcek, S.
Period: 2002-2004
Grant VEGA, No. 1/9014/02

Epizootiological and genetic charcterization of pestivirus infections.
Period: 1999-2001
Project leader: Vilcek, S.
Grant VEGA, No. 1/6215/99

Genetic variability of pestiviruses and their detection at the genetic level
Period 1996-1998
Project leader: Vilcek, S.
Grant VEGA, No. 1/3001/96

The most significant achievements:

Several original procedures for the preparation of 99mTc-radiopharmaceuticals (5 patents)
Development of 99mTc-sublimation generator (2 patents)
Characterization of 2´-phosphodiestrease activity in sera of domestic animals (int. priority)
Development of PCR method for the detection of BHV-1 (int. priority) (PCR primers)
Selection of pan-pestivirus primers (widely used in many international laboratories) (PCR primers)
Development of a nested RT-PCR method for the detection of BRSV (int. priority) (PCR primers)
Significant contribution to molecular epidemiology of CSF
Identification of cINs insertions in cp field BVDV strains
Genetic characterization of the broad collection of ovine pestiviruses
Characterization of 3’-UTR of the pestivirus genome
Genetic variability of BVDV-1 strains (evidence for 11 genetic groups)
Introduction of RT-PCR assay in eradication program against BVD/MD
The first identification of BVDV-2 in cattle in France, Austria and Slovakia
Participation on the first identification of BDV-4 pestivirus genotype (a pestivirus isolated from chamois)
Participation on the first identification of a novel pestivirus isolated from pronghorn antelope
Participation on the first detection and typing of PCV2 in Slovakia
Participation on the first genetic characterization of PRRSV isolates in Slovakia (identification of unusual length polymorphism of ORF7)

Seven Czechoslovak patents
Fellowship (12 months) provided by Scottish Department for Agricultural and Fisheries
Fellowship (3 months) provided by The Fulbright Commission
Grant (36 months) provided by The Wellcome Trust
Several invitation for scientific research in The National Veterinary Institute in Uppsala
Participation on FP5 and FP6 project
Organization of ESVV workshop”Application of molecular-genetic methods in veterinary diagnostic virology”, Kosice, Slovakia, May 27-29, 2008. (Photo)
Participation on the establishment of "INFEKTZOON – Center of excellence for infectious diseases and zoonosis" at University of Veterinary Medicine in Kosice ( Brochure )

Teaching activities:

Special lectures on the application of molecular-genetic techniques in laboratory diagnosis of infectious disease of domestic animals
Lectures from the subject "Principles of Genetic Engineering" at The University of Natural Sciences in Kosice (Thesis)

Selected presentations on international conferences

VILCEK, Š., VRTIAK, O.J., GDOVINOVA, A.: Degradation of adenylyl (2´-5´) adenosine (A2´p5´A in chicken serum. Abstracts of 16th FEBS Meeting, Moskow,1984, p.415.
VILCEK, Š., HERRING, A., NETTLETON, P.: Detection of bovine herpesvirus 1 (BHV 1) sequences by PCR assay. Abstracts of IX th International Congress of Virology, Glasgow, 1993, p.246.
VILCEK, Š., NETTLETON, P.F., HERRING, A.J., ELVANDER, M., BALLAGI-PORDÁNY, A., BELÁK, S.: Detection of BHV-1, BVDV and BRSV in clinical samples by PCR. Abstracts from Third Congress of the European Society for Veterinary Virology, Interlaken, 1994, W5-1.
VILCEK, Š., NETTLETON, P.F., PATON, D.J., BELAK, S.: Molecular characterization of ovine pestiviruses. In.: Proc. Third ESVV Symposium on Pestivirus Infections. Lelystad 19-20 September 1996, (Eds: Edwards, S., Paton, D.J., Wensvoort, G.), CVL Weybridge (UK), 1997, pp.104-107.
VILCEK, Š., STADEJEK, T., BALLAGY-PORDÁNY, A., LOWINGS, J.P., PATON, D.J., BELÁK, S.: Genetic variability of classical swine fever virus. Abstracts of Xth International Congress of Virology, Jerusalem, 1996, PW17-23, p.147.
BELAK, S., VILCEK, Š., EGYED, L., BALLAGI-PORDÁNY, A., BARTHA, A.: Comparative nucleotide sequence analysis of PCR amplicons from animal adenoviruses. Abstracts of Xth International Congress of Virology, Jerusalem, 1996, PW17-4, p.144.
ELVANDER, M., VILCEK, Š., UTTENTHAL, A., BALLAGI-PORDÁNY, A., BELÁK, S.: Antigenic and genetic heterogeneity of G attachment protein of bovine respiratory syncytial virus (BRSV) strains. Abstracts of Xth International Congress of Virology, Jerusalem, 1996, WO4B-7, p.16.
VILCEK, Š., BELÁK, S.: Organisation and diversity of the 3´-noncoding region of classical swine fever virus genome. Programme and Abstracts. 4th International Congress of Veterinary Virology, Edinburgh, 24-27 August 1997, p.50.
STADEJEK, T., VILCEK, Š., PEJSAK, Z., BELAK, S.: Genetic analysis of multiple genome fragments improves the phylogenetic resolution of closely related strains of classical swine fever virus. Programme and Abstracts. 4th International Congress of Veterinary Virology. Edinburgh, 24-27 August 1997, p.188.
VILCEK, Š., NETTLETON, P., BELAK, S.: The use of 5´-noncoding region (5´NCR) for practical genetic detection and analysis of pestiviruses. Abstracts of European symposium on control BVD-virus infection in Cattle. Lillehammer (Norway), Sept., 3-5, 1997, p.15.
VILCEK, Š., PATON, D.J., NETTLETON, P.F., BELAK, S.: Genetic analysis of pestiviruses at the 3´end of the genome. Abstracts from Fifth International Symposium on Positive Strand RNA Viruses. St. Petersburg, Florida, May 23-28, 1998, p. P1-13.
VILCEK, Š., BELAK, S.: Genetic discrimination between vaccine and field strains of classical swine fever virus. Abstracts of OIE Symposium on classical swine fever (hog cholera), Birmingham, 9-10 July 1998, p.22.
VILCEK, Š., GREISER-WILKE, I., NETTLETON, P., PATON, D.: Cellular insertions in the NS2-3 genomic region of BVDV strains. Abstracts from ESVV 4th Pestivirus Meeting, Giessen (Germany), 15-19 March 1999, p S5-3.
VILCEK, Š., ALENIUS, S., PATON, D., BELAK, S.: Herd-specific identification of BVDV in serum of persistently infected cattle. Abstracts from ESVV 4th Pestivirus Meeting, Giessen (Germany), 15-19 March 1999, p. P1-15.
VILCEK, Š., DURKOVIC, B., STROJNY, L., ROSMANITH, W., LOITSCH, A., MOUSSA, A., SCICLUNA, M.T., LANZA, I., PATON, D.: Genetic typing and vatiability of BVDV in Europe. Abstracts from XI. International Congress of Virology, Sydney, 9-13 August 1999, VP66.15, p. 239.
VILCEK, Š., PATON, D.J., DURKOVIC, B., STROJNY, L., IBATA, G., MOUSSA, A., LOITSCH, A., ROSSMANITH, W., VEGA, S., SCICLUNA, M.T., PALFI, V.: BVDV-1 can be separated into at least eleven genetic groups. In: Proc. of the 5th International Congress of the European Society for Veterinary Virology, 27-30 August 2000, Brescia (Italy), pp. 373-374.
VILCEK, Š., PATON, D.J.: A RT-PCR assay for the recognition of border disease virus. In: Proc. of the 5th International Congress of the European Society for Veterinary Virology, 27-30 August 2000, Brescia (Italy), pp. 371-372.
VILCEK, Š., DURKOVIC, B., DEUTZ, A., ROSSMANITH, W., RIDPATH, J., GREISER-WILKE, I., PATON, D.J.: Genetic variability of BVDV strains and search for cellular insertions in the pestivirus genome. In:Proc. „Fruchtbarkeit und Neonatologie“, 28-30 September 2001, Alpbach (Austria), p.53-55.
NETTLETON, P., VILCEK, Š.: Detection of pestiviruses in bovine serum. Abstracts from international symposium „Pestivirus contaminats of bovine sera and other bovine viruses contaminations“. Paris, 29-30 March 2001, p. 33.
VILCEK, Š., RIDPATH, J.F., VAN CAMPEN, H., CAVENDER, J.: Characterization of a novel pestivirus genotype isolated from antelope. Abstracts from Sixth International Symposium on Positive Strand RNA Viruses, Paris, May 28 - June 2, 2001, P2-74.
RIDPATH, J.F., VILCEK, Š., NEILL, J.D., BOLIN, S.B., CARMAT, S.: Mutations in the 5´and 3´regions flanking the NS2 gene found in noncytopathic virulent type 2 bovine viral diarrhea viruses. Abstracts from 20th Annual Meeting of American Society for Virology, University of Wisconsin, Madison, July 21-25, 2001, W18-5.
VILCEK, Š., KOLESAROVA, M., PATON, D.J.: Genetic variability in the 3´-untranslated region of BVDV. Abstracts from 5th Pestivrus Symposium, St. John´s College, Cambridge, UK, 26- 29 August 2002, p. 103.
NETTLETON, P., ALEXANDER, K., MALEY, M., GILRAY, J., VILCEK, Š.: NASBA for detecting pestiviruses. Abstracts from 5th Pestivrus Symposium, St. John´s College, Cambridge, UK, 26-29 August 2002, p. 36.
MAHONY, T.J., McCARTHY, F.M., GRAVEL, J.L., CORNEY, B.C., YOUNG, P.L., VILCEK, S.: Genetic typing of Australian BVDV-1 isolates. In: Proc. of 6th Inter. Congress of Veterinary Virology. 24-27th August 2003, Saint Malo, France, p. 135.
VILCEK, S., Van RIJN, P.A.: Variation in antigenic domains on E2 of CSFV isolates belonging to different phylogenetic groups. In: Proc. of 6th Inter. Congress of Veterinary Virology. 24-27th August 2003, Saint Malo, France, p. 147.
VILCEK, S., ARNAL, M.C., FERNANDEZ-De-LUCO, D., RIBA, L., MALEY, M., GIRLAY, J., WILLOUGHBY, K., NETTLETON, P.F.: A novel pestivirus associated with deaths in Pyrenean chamois. Abstracts from Seventh International Symposium on Positive Strand RNA Viruses. 27 May - 1 June 2004, San Francisco, USA, p. 93.
VILCEK, S., DURKOVIC, B., KOLESAROVÁ, M., PATON, D.J., MISHRA, N., MAHONY, T.: Genetic diversity of BVDV: consequences for classification and molecular epidemiology. Abstracts of Second European Symposium BVDV Control. October 20-22, 2004, Porto, Portugal, p. 15.
VILCEK, S., KOLESAROVA, M., JACKOVA, A.. Genetic heterogeneity of Pestiviruses - Genotypes and Subgenotypes. Abstracts of the XIII International Congress of Virology. July 23 - 28, 2005, San Francisco, p. 174.
VILCEK, S., KOLESAROVA, M., JACKOVA. A.: Genetic diversity of BVDV isolates in European countries. Abstracts from 6th Pestivirus Symposium. Thun, Switzerland, 13-16 September 2005, T6-7, p. 60.
VILCEK, S., KOLESAROVA, M., JACKOVA, A.: Genetic diversity of of bovine viral diarrhoea virus 1 (BVDV-1) genome. Late breaking Abstracts from 20th IUBMB International Congress of Biochemistry and Molecular biology and 11th FAOBMB Congress. Kyoto, Japan, June 18-23, 2006, 4P-A-507, p. 23.
VILCEK, S., WILLOUGHBY, K., NETTLETON, P.: The chamois pestivirus: Further characterisation of the genome. Proceedings of 7th International Congress of Veterinary Virology, Lisabon, Portugal, 24th - 27th September 2006, p. 91.
VILCEK, S., JACKOVA, A., NOVACKOVA, M., PELLETIER, C., AUDEVAL, C., GUENEAU, E., HAFFAR, A., PETIT, E., REHBY, L.: Genetic diversity of French BVDV-1 isolates: Identification of additional subtype. Proceedings of 7th International Congress of Veterinary Virology, Lisabon, Portugal, 24th - 27th September 2006, p. 157.
VILCEK, S., JACKOVA, A., NOVACKOVA, M., KOLESAROVA, M., BENETKA, V., KLEIN, D., MOSTL, K.: Genetic analysis of S1 subunit of bovine coronavirus isolates originating from Central Europe. Abstracts from Eighth International Symposium on positive-strand RNA viruses. Washington DC, USA, 26th - 30th May 2007, P2-F4, p. 85.
VILCEK, S., JACKOVA, A., NOVACKOVA, M.: BVDV-2 in European Union and genetic analysis of BVDV-2 isolate orginating from Slovakia. Middle European bujatric congress, Gura Humoruluj, Suceava, Romania, 5th June - 8th June 2007, Revista Romana de Medicina Veterinara, 17, 2007, p. 61.
VILCEK, S., NETTLETON, P.F.: How many pestiviruses circulate in nature? Pestiviruses in free-living animals: Present status and problems. Proceedings of 13th International World Association of Veterinary Laboratory Diagnosticians Symposium, Melbourne, Australia 11-14 November 2007, p. 33.
VILCEK, S., WILLOUGHBY, K., NETTLETON, P.F.: Genetic characterization of border disease virus isolated from chamois. Proceedings of 13th International World Association of Veterinary Laboratory Diagnosticians Symposium, Melbourne, Australia 11-14 November 2007, p. 222.
VILCEK, S., NOVACKOVA, M., JACKOVA, A., KOLESAROVA, M.: BVDV-2 in Europe and genetic analysis of the isolate originanting from Western Slovakia. In: Proc. of 7th ESVV Pestivirus Symposium, Uppsala, September 16-19 2008, p. 158.
SANDVIK, T., GREISER-WILKE, VILCEK, S.: Towards standardised protocols for genotyping of ruminat pestiviruses? In: Proc. of 7th ESVV Pestivirus Symposium, Uppsala, September 16-19 2008, p. 160.
VILCEK, S., NOVACKOVA, M., JACKOVA, A.: Detection of PCV-2 using real-time PCR based on the application of LUX fluorogenic primer (LUX PCR). In: Poster Proceedings IPVS 2008. 20th International Pig Veterinary Society Congress, Durban, South Africa, 22-26 June 2008, P01.092, p. 92.
VILCEK, S., NOVACKOVA, M., JACKOVA, A., PISTL, J.: Genetic analysis of porcine circovirus type 2 (PCV2) isolates originating from Central Europe. In: Abstract book from XIV. International Congress of Virology, 10-15 August 2008, Istanbul, Turkey, p. 381.
JACKOVA, A., NOVACKOVA, M., VILCEK, S.: Phylogenetic analysis of porcine reproductive and respirátory syndrome virus isolates from Slovakia. Programme and Proceedings of 8th International Congress of Veterinary Virology, Budapest, 23-26 August 2009, p. 146.
VILCEK, S., WILLOUGHBY, K., NETTLETON, P.F., BECHER, P.: Genome seqence of a border disease virus strain isolated from a Pyrenean chamois. Programme and Proceedings of 8th International Congress of Veterinary Virology, Budapest, 23-26 August 2009, p. 236.
VILCEK, S., JACKOVA, A., VLASAKOVA, M., LESKOVA, V.: Genetic analysis and typing of PRRSV isolates in ORF5 and ORF7 originating from pig farms in Central Europe. Proceedings of the 21st IPCV Congress, Vancouver, Canada, July 18-21, 2010. p. 502.
VILCEK, S., JACKOVA, A., LESKOVA, V., VLASAKOVA, M.: Genetic analysis of PRRSV isolates from pig farms in Slovakia. In Proceeding EuroPRRS2011 "Undestanding and combating PRRS in Europe", Novi Sad, 12th – 14 th October 2011, p. 44-46.

Selected references:

VILCEK, S., MACHAN, V., KALINCAK, M., NICAK, A.: ^99mTc-Ti-DTPA: Preparation, control and biological distribution. Int. J. Appl. Rad. Isotop., 30, 1979, p. 673-676. (PubMed)
VILCEK, S., KALINCAK, S., MACHAN, V.: ^99mTc-labelled compounds prepared with sodium dithionite as reducing agent. Nucl. Med. (Stuttg.), 20, 1981, p. 283-289. (PubMed)
VILCEK, S., MACHAN, V., KALINCAK, M.: ^99mTc-labelled compounds prepared with antimony (III) as reducing agent. Radiobiol. Radiother., 23, 1982, p. 333-338. (PubMed)
VILCEK, S., MACHAN, V., KALINCAK, M.: ^99mTc-labelled compounds prepared with molybdenum (III) as reducing agent. Int. J. Appl. Rad. Isot., 35, 1984, p. 228-230. (PubMed)
VILCEK, S., KALINCAK, M., MACHAN, V.: ^99mTc-labelled compounds prepared with tungsten (III) as reducing agent. J. Radioanal. Chem., 88, 1985, p. 359-367.
VILCEK, S., VRTIAK, O. J., SMRT, J., JUROVCIK, M.: Stability of (2´-5´)oligoadenylates in varios sera. FEBS Lett., 166, 1984, p. 311-313. (PubMed)
VILCEK, S., VRTIAK, O. J., JUROVCIK, M., SMRT, J.: Hydrolysis of adenylyl (2´-5´) in chick sera. Biochem. Int., 12, 1986, p. 949-956. (PubMed)
VILCEK, S., DELIOVA, I., FORGAC, O., STROJNY, L., TAKACSOVA, I., HARVAN, M., BENKO, G.: Detection of bovine herpesvirus 1 with various types of DNA probes. Acta. Vet. Hung., 41, 1993, p. 179-190. (PubMed)
VILCEK, S., DELIOVA, I., STROJNY, L., FORGAC, O., HARVAN, M.: The effect of the mode of sampling on BHV-1 detection in infected cattle by dot-blot hybridisation. Vet. Microbiol., 36, 1993, p. 355-358. (PubMed)
VILCEK, S.: Detection of the bovine herpesvirus-1 (BHV-1) genome by PCR. J. Virol. Meth., 41, 1993, p. 245-248. (PubMed)
VILCEK, S., HERRING, A.: A single tube method for the preparation of digoxigenin-labelled probe from RNA by PCR. Biotechniques, 16, 1994, p. 1020. (PubMed)
VILCEK, S., HERRING, A. J., HERRING, J. A., NETTLETON, P. F., LOWINGS, J. P., PATON, D. J.: Pestiviruses isolated from pigs, cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis. Arch. Virol., 136, 1994, p. 309-323. (PubMed)
VILCEK, S., ELVANDER, M., BALLAGI-PORDANY, A., BELAK, S., Development of nested PCR assays for detection of bovine respiratory syncytial virus in clinical samples. J. Clin. Microbiol., 32, 1994, p. 2225-2231. (PubMed)
VILCEK, S., NETTLETON, P. F., HERRING, J. A., HERRING, A. J.: Rapid detection of bovine herpesvirus 1 (BHV-1) using the polymerase chain reaction. Vet. Microbiol., 42, 1994, p. 53-64. (PubMed)
EASTON, L. A., VILCEK, S., NETTLETON, P. F.: Evaluation of a "one tube" reverse transcription-polymerase chain reaction for the detection of ruminant pestiviruses. J. Virol. Meth., 50, 1994, p. 343-348. (PubMed)
PATON, D. J., CARLSSON, U., LOWINGS, J. P., SANDS, J. J., ALENIUS, S., VILCEK, S.: Identification of herd-specific bovine viral diarrhoea virus isolates from infected cattle and sheep. Vet. Microbiol., 43, 1995, p. 283-294. (PubMed)
VILCEK, S., NETTLETON, P. F., HERRING, A. J.: Detection of bovine herpesvirus 1 in clinical samples by the polymerase chain reaction. Dtsch. Tierärztl. Wschr., 102, 1995, p. 219-258. (PubMed)
LYAKU, J. R. S., VILCEK, S., NETTLETON, P. F., MARSDEN, H. S.: The distinction of serologically related ruminant alphaherpesviruses by the polymerase chain reaction (PCR) and restriction endonuclease analysis. Vet. Microbiol., 48, 1996, p. 135-142. (PubMed)
VILCEK, S., BELAK, S.: Genetic identification of pestiviruses strain Frijters as a border disease virus from pigs. J. Virol. Meth., 60, 1996, p. 103-108. (PubMed)
VILCEK, S., STADEJEK, T., BALLAGI-PORDANY, A., LOWINGS, J. P., PATON, D. J., BELAK, S.: Genetic variability of classical swine fever virus. Virus Res., 43, 1996, p. 137-147. (PubMed)
VILCEK, S., STADEJEK, T., TAKACSOVA, I., STROJNY, L., MOJZIS, M.: Genetic analysis of classical swine fever virus isolates from a small geographic area. Dtsch. Tierärztl. Wschr., 104, 1997, p. 9-12. (PubMed)
VILCEK, S., NETTLETON, P. F., PATON, D. J., BELAK, S.: Molecular characterisation of ovine pestiviruses. J. Gen. Virol., 78, 1997, p. 725-735. (PubMed)
VILCEK, S.: Secondary structure of the 5´-noncoding region of border disease virus genome RNAs. Vet. Med.-Czech, 42, 1997, p. 125-128. (PubMed)
PATON, D., GUNN, M., SANDS, J., YAPP, F., DREW, T., VILCEK, S., EDWARDS, S.: Establishment of serial persistent infections with bovine viral diarrhoea virus in cattle and sheep and changes in epitope expression related to host species. Arch. Virol., 142, 1997, p. 929-938. (PubMed)
VILCEK, S., BELAK, S.: Organisation and diversity of the 3´-noncoding region of classical swine fever virus genome. Virus Genes, 15, 1997, p. 181-186. (PubMed)
STADEJEK, T., VILČEK, Š., LOWINGS, P., BALLAGI-PORDÁNY, A., PATON, D., BELÁK, S.: Genetic heterogeneity of classical swine fever virus in Central Europe. Virus Res., 52, 1997, 195-204. (PubMed)
VILČEK, Š., BJORKLUND, H. V., HORNER, G. W., MEERS, J., BELÁK, S.: Genetic typing of pestiviruses from New Zealand. New Zeal. Vet. J., 46, 1998, 35-37.
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HYNDMAN, L., VILČEK, Š., CONNER, J., NETTLETON, P.F.: A novel nested reverse transcription PCR detects bovine viral diarrhoea virus in fluids from aborted bovine foetuses. Virol. Meth., 71, 1998, 69-76. (PubMed)
VILČEK, Š., BELÁK, S.: Classical swine fever virus: Discrimination between vaccine strains and European field viruses by restriction endonuclease cleavage of PCR amplicons. Acta Vet. Scand., 39, 1998, 395-400. (PubMed)
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ELVANDER. M., VILČEK, Š., BAULE, C., UTTENTHAL, A., BALLAGI-PORDANY, A., BELÁK S.: Genetic and antigenic analysis of the G attachment protein of bovine respiratory syncytial virus strains. J. Gen. Virol., 79, 1998, 2939-2946. (PubMed)
VILČEK, Š., PATON, D., MINOR, P., BENTLEY, M.: No confirmation of pestivirus RNA in human virus vaccines. J. Clin. Microbiol., 37, 1999, 1653. (PubMed)
VILČEK, Š., PATON, D., LOWINGS, P., BJORKLUND, H., NETTLETON, P., BELÁK, S.: Genetic analysis of pestiviruses at the 3'end of the genome. Virus Genes, 18, 1999, 107-114. (PubMed)
VILČEK, Š., ALENIUS, S., PATON, D. J., MITTELHOLZER, C., BELÁK, A.: Genetic clustering of bovine viral diarrhoea viruses in cattle farms: Genetic identification and analysis of viruses directly from cattle sera. Vet. J., 158, 1999, 33-38. (PubMed)
BJORKLUND, H., LOWINGS, P., STADEJEK, T., VILČEK, Š., GREISER-WILKE, I., PATON, D.: Phylogenetic comparison and molecular epidemiology of classical swine fever virus. Virus Genes, 19, 1999, 189-195. (PubMed)
VILČEK, Š., DREW, T. W., McGOLDRICK, A., PATON, D. J.: Genetic typing of bovine pestiviruses from England and Wales. Vet. Microbiol., 69, 1999, 227-237. (PubMed)
VILCEK, Š., PATON, D.J., ROWE, L.W., ANDERSON, E.C.. Typing of pestiviruses from eland in Zimbabwe. J. Widlife Dis., 36, 2000, 165-168. (PubMed)
VILCEK, Š. , PATON, D.J.: A RT-PCR assay for the rapid recognition of border disease virus. Vet. Res., 31, 2000, 437-445. (PubMed)
VILCEK, Š., GREISER-WILKE, I., NETTLETON, P., PATON, D.J.: Cellular insertions in the NS2-3 genome region of cytopathic bovine viral diarrhoea virus (BVDV) isolates. Vet. Microbiol., 77, 2000, 129-136. (PubMed)
VILCEK, Š., PATON, D.J., ĎURKOVIČ, B., STROJNÝ, L., IBATA, G., MOUSSA, A., LOITSCH, A., ROSSMANITH, W., VEGA, S., SCICLUNA, M.T., PÁLFI, V.: Bovine viral diarrhoea virus genotype 1 can be separated into at least eleven genetic groups. Arch. Virol., 146, 2001, 99-115. (PubMed)
VILCEK, Š., STROJNÝ, L., ĎURKOVIČ, B., ROSSMANITH, W., PATON, D.: Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method. J. Virol. Methods, 92, 2001, 19-22. (PubMed)
ROSSMANITH, W., VILCEK, Š., WENZL, H., ROSSMANITH, E., LOITSCH, A., ĎURKOVIČ, B., STROJNÝ, L., PATON, D.J.: Improved antigen and nucleic acid detection in a bovine virus diarrhoea eradication program. Vet. Microbiol., 81, 2001, 207-218. (PubMed)
VILCEK, Š.: Identification of pestiviruses contaminating cell lines and fetal calf sera. Acta Virol., 45, 2001, 81-86. (PubMed)
VILCEK, Š., GREISER-WILKE, I., ĎURKOVIČ, B., OBRITZHAUSER, W., DEUTZ, A., KOFER, J.: Genetic diversity of recent bovine viral diarrhoea viruses from the Southern of Austria (Styria). Vet. Microbiol., 91, 2003, 285-291. (PubMed)
VILCEK, Š., DURKOVIC, B., BOBAKOVA, M., SHARP, G., PATON, D.J.: Identification of bovine viral diarrhoea virus 2 in cattle in Slovakia.
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VILCEK, Š., GREISER-WILKE, I., DURKOVIC, B., OBRITZHAUSER, W., DEUTZ, A., KOFER, J.: Genetic diversity of recent bovine viral diarrhoea viruses from the Southern of Austria (Styria).
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VILCEK, Š., MOJZISOVÁ, J., BAJOVA, V., PAULIK, Š., STROJNY, L., DURKOVIC, B., HIPIKOVA, V.: A survey for BVDV antibodies in cattle farms in Slovakia and genetic typing of BVDV isolates from imported animals. Acta Vet. Hung., 51, 229-236, 2003. (PubMed)
NETTLETON, P.F., GILRAY, J.A., CALDOW, G., GIDLOW, J.R., DURKOVIC, B., VILCEK, Š.: Recent isolates of bovine respiratory syncytial virus from Britain are more closely related to isolates from USA than to earlier British and current mainland European isolates. J. Vet. Med. B., 50, 2003, 196-199. (PubMed)
VILCEK, Š., NETTLETON, P., PATON, D.: Remarkable cross-reaction of pan-pestivirus PCR primers with poliovirus genome. J. Virol. Methods, 114, 2003, 167-170. (PubMed)
VILCEK, Š., DURKOVIC, B., KOLESAROVA, M., GREISER.WILKE, I., PATON, D.: Genetic diversity of international bovine viral diarrhoea virus (BVDV) isolates: identification of a new BVDV-1 genetic group. Vet. Res. 35, 2004, p. 609-615 (PubMed)
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RIDPATH, J.R., NEILL, J.D., VILCEK, S., DUBOVI, E.J., CARMAN, S.: Multiple outbreaks of severe acute BVDV in North America occuring between 1993 and 1995 linked to the same BVDV2 strain. Vet. Microbiol., 114, 2006, p.196-204. ( PubMed)
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The most frequently cited paper:

Citation for all papers:1380, 2 papers cited more than 100 times, H index = 20 (all data are without self-citations)

PubMed

(This paper is widely used for panpestivirus primers - 324 and 326 - selected from 5´-UTR of the pestivirus genome. Primers have been used not only in Slovakia but also in Argentina, Austria, Germany, Italy, Japan, Poland, Sweden, The Czech Republic, UK, USA, etc.)

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